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α-氨基酸的重氮化取代质子化

2024-01-26 12:18:50

illed aq. NaNO2 (20mmol, 2.0eq) was added as drops to this solution and temperature was controlled below 0°C and stirred for 5h in ice bath. The ice bath was removed and the solution was stirred overnight, extracted with

EtOAc (3×25mL) at highly acidic pH. The organic extract was concentrated which afforded yellow oily products 12a–d which was further concentrated under N2.4.4.3 (2S)-2-Chloro-4-methylpentanoic acid 12c Colorless oil (0.77 mL, 0.83 g, 80percent)

【Saddiqa, Aisha; Raza, Abdul R.; Black, Did Stc.; Kumar, Naresh; Tetrahedron Asymmetry ; vol. 25; nb. 9; (2014); p. 736 -743】

In a 3-necked 1000 ml. RB flask, sodium nitrite (68.4 g, 0.99 mol) was added in small batches to the aqueous solution of a mixture of DL-serine (52.4 g, 0.50 mol), potassium chloride (130.4 g, 1.75 mol) and HCl (116.0 g of 36.5percent-38percent w/v aq. sol., 1.21 mol) (taken together in 490 mL of water) at 0° C.-10° C. After complete addition, reaction mixture brought to room temperature and kept overnight for the reaction. Solution turned from clear and off-white to clear and light green. The product was salted out with NaCl and extracted with 5 times of 100 mL of ethyl acetate. Organic phase was washed 5 times with saturated NaCl aqueous solution (50 mL each) and then dried over anhy. Na2SO4. Solution was filtered and solvent was evaporated by trap-to-trap distillation method followed by drying in the vacuum chamber.

Product was recrystallized in CH2Cl2. Yield=36.5 g (58percent).

To a solution of compound 20 (200 mg, 1 mmol) in 5 mL of pyridinium poly(hydrogen fluoride) is slowly added, with good stirring, sodium nitrite (105 mg, 1.5 mmol). After being stirred at room temperature for 18 h, the water (20 mL) is added. The resulted solution is extracted with ether. The ether layer is washed with 1N NaOH. The aqueous is acetified with HCl and extracted with ether. The ether layer is dried (Na2SO4). The solvent is removed in vacuo.

The crude product 22 (is used for next step without further purification).

【US2011/65724; (2011); (A1) English】

L-isoleucine (10.0 g, 76.2 mmol) was dissolved in 1 M H2SO4 (200 mL) and the solution cooled to ~0 °C using an ice-water bath. Sodium nitrite (42.1 g, 610 mmol) was dissolved in water (200 mL) and then slowly added to the cold stirred amino acid solution over 4 hours. The reaction was allowed to warm to ambient temperature and the mixture continued to stir for 18 h. The aqueous solution was then saturated with sodium chloride, transferred to a separation funnel and extracted with EtOAc (3×75 mL). The combined organic layers were washed with water (2 × 20 mL) and brine (2×20 mL), dried over anhydrous sodium

sulfate. Removal of solvent under reduced pressure ge D in ~99percent (9.97 g)

【Banasik, Brent A.; Wang, Lee; Kanner, Arielle; Mikael Bergdahl; Tetrahedron ; vol. 72; nb. 19; (2016); p. 2481 - 2490】

General procedure: The Boc-protected amino acid (1 mmol) was treated with TFA (3 mL) for 15 minutes. Afterevaporation the residue was dissolved in dioxane-water (1:1, 4 mL) and the flask wasplaced in an ice bath. tert-Butylnitrite (0.13 mL, 1.1 mmol) was added and stirring wasmaintained under nitrogen at room temperature for one hour. After pouring the reactionmixture onto celite and evaporation (50 Pa, 30 °C) into a dry free-floating powder,separation was performed utilizing a CombiFlash Rf (Teledyne

ISCO) automated flashchromatography apparatus by means of AcOH-MeOH-EtOAc (1:9:90) on a normal phasesilica column affording the pure a-hydroxy carboxylic acid in the yield specified in Table 1.HO-His(Bom)-OH and HO-Arg(Tos)-OH were isolated by adding water (30 mL) to thereaction mixture followed by freeze drying and HPLC purification.

【Stuhr-Hansen, Nicolai; Padrah, Shahrokh; Strømgaard, Kristian; Tetrahedron Letters; vol. 55; nb. 30; (2014); p. 4149 -4151】

To a solution of (S)-(4-nitrophenyl) glycine (10 g, 47.6 mmol) in a mixture of water (50 mL), H2SO4 (1M, 60 mL) and acetone (150 mL) at -5° C., was added under stirring, a solution of sodium nitrite (9.85 g, 142.8 mmol) in water (40 mL) dropwise over a period of 30 min. The reaction mixture was stirred at -5 to 0° C. for another 1.5 h, followed by stirring at room temperature for 16 h. Acetone was removed and then the reaction mixture was diluted with 500 mL ethyl acetate. Organic layer was washed with brine, dried over anhydrous Na2SO4, and concentrated. The crude mass was purified by crystallization from

isopropyl acetate (9.0 g, 96percent)

A solution of (S)-2-aminopent-4-enoic acid (5.0 g,43.4 mmole, 1.0 eq.) in water/acetic acid (100 mE, 8/2 v/v) is added a solution of NaNO2 (7.5 g, 108.5 mmole, 2.5 eq.) in distilled water (20 mE) over 30 minutes period at 0° C. The resulting solution is stirred 2 hours at 0° C., then 12 hours at ambient temperature. The solution is then cooled down to 0° C. again, quenched with a

solution of CH3NH2 in THF (2 M, 18.2 mE, 36.4 mmole, 2.0 eq.). THF is brieflyevaporated under reduced pressure and the residual aqueous solution is acidified with conc. HC1 to pH 2. This acidic solution is extracted with EtOAc (50 mLx4) and the organic layer is combined, dried over Na2SO4, and then concentrated under reduced pressure. The residue is purified by chromatography over silica gel with mixed solvent system of (CH2C12/MeOH/water, 10/5/0.5, v/v/v). The title compound is obtained

in 4.7 g (40.4 mmole, 93percent) as light brown gel